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Column:

Target -NH2( 5 μm, 4.6 x 250 mm )

Mobile phase:

H2O : ACN= 25 : 75 ( v/v )

Flow rate:

1.0 mL/min

Temperature:

35 ℃

Detection:

ELSD ( 60 ℃,2 mL/min )

Injection volume:

5 μL

Sample:

Sucrose ( 1.28 mg/mL ), fructose ( 1.37 mg/mL ) and glucose ( 0.52 mg/mL )

Column:

Target -NH2( 5 μm, 4.6 x 250 mm )

Mobile phase:

ACN : H2O = 70 : 30 ( v/v )

Flow rate:

1.0 mL/min

Temperature:

35 ℃

Detection:

RI 30℃

Injection volume:

20 μL

Sample:

sample ( 10.0 mg/mL )